iNOS expression in the retina and iNOS modulation of retinal neovascularization. iNOS^–/– retinas were stained with NOS isoform-specific antibodies. In panels A-C, red depicts agglutinin staining for vessel visualization and green represents NOS antibody positive regions. Panel A is an iNOS^–/– retina stained with the iNOS-specific antibody. Panels B and C are iNOS^–/– stained with eNOS isoform-specific antibody. The iNOS.gfp chimeric mice underwent the retinal ischemia model followed by perfusion with TRITC before eye enucleation and confocal imaging of the retinas. Panels D through G are red and green channel merged images from control (D,F) and treated (E,G) retinas. Original magnification × 60; size bar about 10 μm.