MECA-32⁺, gfp⁺, HSC-derived endothelial cells in eNOS.gfp spleens. Splenic cryosections were prepared from C57BL/6.gfp (A-D), iNOS.gfp (E-H), and eNOS.gfp (I-P) chimeras. Sections were stained with anti-MECA-32 antibody and a Texas red-conjugated secondary antibody to delineate vascular endothelium. Sections were mounted with DAPI (A,E,I,M) to delineate nuclei with blue fluorescence, examined for gfp expression (B,F,J,N) via green fluorescence, or MECA-32 staining (C,G,K,O) via red fluorescence. (D,H,L,P) Merged images of the DAPI, gfp, and MECA-32 Texas red stains. Original magnifications × 64 (A-L) and × 32 (M-P).