Influence of extracellular ATP on IL-12, IL-23, and IL-27 expression of E coli–activated MoDCs. MoDCs were left untreated or were incubated with E coli in the absence or presence of ATP (250 μM). (A) Secreted IL-12p40 and (B) IL-12p70 levels were analyzed by ELISA 2 days after stimulation. (C) Expression of IL-23p19, (D) IL-27p28, and (E) EBI3 was analyzed by RT-qPCR 14 to 18 hours after stimulation. Data represent mean ± SEM of 5 to 6 separate experiments.