Influence of various P2 receptor agonists on IL-12, IL-23, and IL-27 expression. MoDCs were activated with E coli in the absence or presence of ATP, ADP, UDP, ATPγS, or AR-C67085 (P2Y₁₁ receptor agonist) at the indicated concentrations. (A) IL-12p40 and (B) IL-12p70 levels were determined by ELISA. Cytokine production in the absence of nucleotides was normalized to 100%. Data are mean values of 7 experiments. (C) IL-23p19, (D) IL-27p28, and (E) EBI3 expression was quantified by RT-qPCR and is expressed as n-fold increase compared with MoDCs left untreated. Data are mean values of 4 experiments.