Figure 2.
Reversal of the NSAID-dependent block in stress kinase activation by PGE2. Immunoblot analysis of postnuclear supernatants from Jurkat cells nonactivated (0) or activated by CD3 (CD3) ligation for 5 minutes, in the presence or absence of 15 μM sc-560, alone or in combination with 1 μg/mL PGE2. Each filter was sequentially probed by immunoblot with antiphospho–JNK or antiphospho–p38 antibodies and then, after stripping, with control antitubulin or anti-p38 antibodies as indicated. The migration of molecular mass markers is shown.
