Figure 4.
Figure 4. Transcriptional clonality assay of G6PD in human CD33+ cells isolated from mice that received transplants of IM PB CD34+ cells. Lanes 1-3 show clonal expression of G6PD in human CD33+ cells from 3 individual mice, each given transplants of CD34+ cells from a different female IM patient: IM-1 (lane 1), IM-2 (lane 2) and IM-3 (lane 3). Lane 4 shows negative control. Lane 5 shows positive control for clonal expression of G6PD on T allele. Lane 6 shows positive control for clonal expression of G6PD on C allele. Lane 7 shows positive control for polyclonal expression of G6PD on both T and C alleles.

Transcriptional clonality assay of G6PD in human CD33+ cells isolated from mice that received transplants of IM PB CD34+ cells. Lanes 1-3 show clonal expression of G6PD in human CD33+ cells from 3 individual mice, each given transplants of CD34+ cells from a different female IM patient: IM-1 (lane 1), IM-2 (lane 2) and IM-3 (lane 3). Lane 4 shows negative control. Lane 5 shows positive control for clonal expression of G6PD on T allele. Lane 6 shows positive control for clonal expression of G6PD on C allele. Lane 7 shows positive control for polyclonal expression of G6PD on both T and C alleles.

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