Figure 5.
Figure 5. Analysis of hematopoietic tissues obtained from NOD/SCID mice receiving transplants of CD34+ cells from patient IM-4. (A) Representative flow cytometric analysis of BM (left column), spleen (middle column), and PB (right column) from mice that received transplants of PB CD34+ cells isolated from patient IM-4. Cells were analyzed by flow cytometry at 12 weeks after transplantation. (B) May-Grunwald-Giemsa staining (× 200) and (C) anti-CD34 immunochemical staining (× 200) of a section of tibia from the mouse that received a transplant of cells isolated from patient IM-4.

Analysis of hematopoietic tissues obtained from NOD/SCID mice receiving transplants of CD34+ cells from patient IM-4. (A) Representative flow cytometric analysis of BM (left column), spleen (middle column), and PB (right column) from mice that received transplants of PB CD34+ cells isolated from patient IM-4. Cells were analyzed by flow cytometry at 12 weeks after transplantation. (B) May-Grunwald-Giemsa staining (× 200) and (C) anti-CD34 immunochemical staining (× 200) of a section of tibia from the mouse that received a transplant of cells isolated from patient IM-4.

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