Effects of arachidonic acid metabolites, MG-132, and troglitazone on cell death and NF-κB activity. HS-Sultan cells were treated with 10 μM PGA₂, PGE₂, PGF_2α, 15d-PGJ₂, 0.25 μM proteasome inhibitor MG-132, 10 μM PPARγ agonist troglitazone (Troglit), or control diluent. (A) After 3 hours, whole-cell lysates were assayed for NF-κB DNA-binding activity by EMSA (top panel). The levels of NF-κB DNA-binding activity were quantified by MDP analysis and expressed as percent of control (bottom panel). (B) At 8 (□), 24 (▦), and 48 hours (▪), apoptosis was evaluated by FACS analysis of annexin V⁺ cells.