Thymus graft restores normal myelopoiesis in nude mice. BALB/cnu/nu mice were grafted with 2 to 3 fetal thymic lobes each under the renal capsule. After 6 to 8 weeks thymus-grafted (ThyG) mice were killed and analyzed. (A) WBC and differential cell counts in normal (□), nude (▪), and ThyG nude (▦) mice peripheral blood. Band neutrophil (Band), segmented neutrophil (Segm), lymphocyte (Lymph), and monocyte (Monoc) counts are shown. (B) Bone marrow morphologic analysis. Neutrophils precursor (Neut Prec), segmented neutrophils (Segm), erythroblast (Erythr), lymphocyte (Lymph), and monocyte (Monoc) counts are shown. Error bars mean SEM. (C) Bone marrow flow cytometry analysis in ThyG mice. Dot plots are representative of SCA-1xCD11b staining. Gates show SCA-1+CD11b+ cells population and respective mean ± SD are indicated. (D) Estimative of erythroid (BFU-E) and myeloid progenitors/precursors (CFU-GM) through clonogenic assays in BALB/c (▪), BALB/cnu/nu (▴), and ThyG (♦) mice. Data are representative of 3 independent experiments. *P < .001; **P < .01. Horizontal bars indicate the mean.
