Responses to RhD autoantigen by PBMCs from patients with CLL with secondary AIHA. Representative experiments showing proliferation by PBMCs from patients CLL+AIHA1 (A), CLL+AIHA2 (B), and CLL+AIHA3 (C) after stimulation with a panel of overlapping 15-mer peptides spanning the sequence of the RhD protein (▪) or purified RhD protein (▨). Control unstimulated cultures were also included (□). Peptides are numbered 1 to 68 from the N-terminus. The dotted line indicates the level of response taken as representing a positive response (SI > 3). Error bars indicate SD.