Figure 5.
Activation of endothelial cells by anti–annexin A2 mAb-derived F(ab′)2 fragments. Endothelial cells were cultured in 96-well microplates and prepared as described in “Materials and methods,” and then incubated for 4 hours with medium, anti–annexin A2 mAb–derived fragments at the designated concentration (▧, Fab fragments; ▪, F(ab′)2 fragments), intact annexin A2 mAb (600 nM), or LPS (1 μg/mL). (A) Adhesion of CMFDA-labeled Mono Mac 6 cells, measured in arbitrary fluorescence units (AFUs). (B) Expression of endothelial cell surface E-selectin, measured by ELISA. Error bars represent the mean plus or minus SEM of quadruplicate points. **P < .001, ***P < .0001 versus medium alone. This experiment is representative of 3 so performed.
