PKCδ is associated with WT and D814Y Kit. IC2 cells expressing WT or D814Y Kit were stimulated with 100 ng/mL murine SCF for 15 minutes at room temperature prior to lysis. (A) Whole cell lysate protein (600 μg per sample) was immunoprecipitated with an antibody specific for Kit, resolved by SDS-PAGE, transferred to immobilon-P, and immunoblotted with an antibody specific for PKCδ. Lysate protein (600 μg) immunoprecipitated with normal goat IgG was included as a negative control and 300 μg lysate protein immunoprecipitated with an antibody specific for PKCδ was included as a positive control. (B) The immunoblots were subsequently stripped and reprobed with an antibody specific for Kit. These data are representative of results from more than 3 experiments.