Morphological changes in light microscopy of cytospin preparations of cultured nascent reticulocytes. Aliquots of cultured nascent reticulocytes were harvested for cytospin preparations at the times shown. Upper row shows 3, 3′-dimethoxybenzidine staining, and lower row shows new methylene blue supravital staining for reticulin. For reference, each field of the 0-hour reticulocytes shows an extruded nucleus (arrows). Magnification, × 450.