Pim-1 is expressed in BaF3/ITD cells but not by FL stimulation of BaF3/FLT3 cells. Comparison of Pim-1 expression in BaF3 cells, FLT3-transformed BaF3 cells, and FLT3/ITD-transformed BaF3 cells treated with or without IL-3, FL, or CEP-701. Cell extracts (100 μg/sample) were resolved by 12% polyacrylamide gel electrophoresis and immunoblotted with anti-Pim-1 antibody. Lanes 1, 5, and 9 have no cytokines or CEP-701 treatment; lanes 2, 6, and 10, IL-3 (1 ng/mL) for 2 hours; lanes 3, 7, 11, FL (10 ng/mL) for 2 hours; lanes 4, 8, 12, IL-3 (1 ng/mL) and 50 nM CEP-701 for 2 hours; and lane 13, 50 nM CEP-701 for 2 hours.