Figure 1.
Immunization of mice with DCs loaded withbcr-abl+leukemia-derived CRCL induces BCR-ABL–specific IFN-γ secretion. Mice were immunized with DCs (5 × 105) loaded with 12B1- or 32Dp210-derived CRCL, or with a BCR-ABL peptide, GFKQSSKAL, or with an irrelevant peptide, HYLSTQSALSK, on days –14 and –7. Control mice were immunized with equal volumes of PBS or equal numbers of empty DCs. On day 0, splenocytes were collected and restimulated with indicated peptide or CRCL for 48 hours. IFN-γ production was determined by ELISPOT assay. Error bars represent SEM.
