GAS6 activates SHP-2. (A) SHP-2 activation by GAS6. Quiescent, confluent ECs were stimulated for the indicated times with GAS6 (1 ng/mL), and cell lysates were immunoprecipitated by Ab anti–SHP-2. Immunodetection was performed with Ab anti–P-Tyr or anti–SHP-2. (B-C) GAS6 mediates SHP-2 association with Axl. Quiescent, confluent ECs were stimulated for 30 minutes with GAS6 (1 ng/mL), and cell lysates were immunoprecipitated by Ab–anti-Axl (B) or anti–SHP-2 (C). (C) Immunoprecipitate was divided into 2 aliquots. Immunodetection was performed as indicated. The results are representative of 3 similar experiments.