Figure 2.
Response of p276-290–reactive T-cell lines in cultures of β2GPI and anionic PL. Autologous immature DCs and macrophages were pulsed with native β2GPI alone, DOPS liposomes, a mixture of native β2GPI and DOPS liposomes, MalBP, or GP-F; induced to maturate or activate; and used to stimulate the p276-290–reactive T-cell lines KS3 and EY3. Synthetic peptides were pulsed with APCs before their coculture with T cells. The antigen-induced T-cell response was measured by [3H]thymidine incorporation. Results are shown as mean and standard deviation. A representative result from at least 2 independent experiments is shown.