American Society of Hematology

Figure 1.

$Figure 1. Absence of NPC tumor cells in nasopharynx after treatment. Biopsies taken before (left) and after (right, representative of 7 biopsies) the administration of EBV-specific CTLs as adjuvant treatment in a patient with refractory NPC (P894) were analyzed for the presence of EBV-positive tumor cells by in situ hybridization for EBER using a digoxigenin/alkaline phosphatase-labeled probe (Sigma-Genosys, The Woodlands, Texas). Stained slides were examined with a Zeiss Microscope (Axioscope, Carl Zeiss, Thornwood, NY) at 200 × magnification using a Pan NeoFluar lens (20 ×/0.50). Images were captured using a Spot Insight camera and software (3.4 P.C.) (Diagnostic Instruments, Sterling, MI).$

Absence of NPC tumor cells in nasopharynx after treatment. Biopsies taken before (left) and after (right, representative of 7 biopsies) the administration of EBV-specific CTLs as adjuvant treatment in a patient with refractory NPC (P894) were analyzed for the presence of EBV-positive tumor cells by in situ hybridization for EBER using a digoxigenin/alkaline phosphatase-labeled probe (Sigma-Genosys, The Woodlands, Texas). Stained slides were examined with a Zeiss Microscope (Axioscope, Carl Zeiss, Thornwood, NY) at 200 × magnification using a Pan NeoFluar lens (20 ×/0.50). Images were captured using a Spot Insight camera and software (3.4 P.C.) (Diagnostic Instruments, Sterling, MI).

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