The glucose pathway is involved in regulating intracellular ROS levels. Intracellular ROS levels were measured by DCF-DA staining (A-C). (A) MO7e.BCR-ABL cells were treated for 18 hours with imatinib mesylate (1 μM) or for 1 hour with either rotenone (1 μM) or phloretin (100 μM) as indicated (n = 3). (B) BaF3.BCR-ABL cells were treated for 18 hours with 2-DOG (4.5 mg/mL) or 3-OMG (4.5 mg/mL) as indicated and compared with cells in the presence of 4.5 mg/mL glucose (shaded histograms). (C) The Philadelphia chromosome-positive cell lines K562, KU812, BV173, and MEG01 were left untreated (shaded histograms) or treated with 2-deoxyglucose (open histograms). (D) Cells from a patient in CML blast crisis were left untreated or treated for 6 hours with 2-deoxyglucose (2 mg/mL), and the change in intracellular ROS was calculated relative to untreated cells (n = 4). The right panel shows the plot of a typical experiment, comparing untreated cells (control) with 2-deoxyglucose (2-DOG)-treated cells. Error bars indicate standard error of the mean. ^*Significant differences (P < .05) were observed between treated and control cells.