Donor-type APCs are sufficient for induction of cGVHD. Combined data from 2 experiments are shown. On day 0, chimeric recipient mice (previously prepared) were lethally irradiated and reconstituted with 8 × 106 WT BM cells alone (broken line; n = 21); or WT BM plus 107 WT spleen cells (host → host) (thin solid line; n = 32); (donor → host) (bold solid line; n = 36). Data for all BM control recipients were combined. (A) Incidence of cGVHD. †P < .01 for donor → host recipients versus host → host recipients of spleen cells. (B) Average clinical disease score for mice affected with cGVHD (unaffected mice are excluded). BM control mice did not get cGVHD and are represented on the graph as scoring “0.” (C) Pathology scores for representative mice. Mean score is indicated by a horizontal bar.
