Phenotypic characterization of TAMs. Purified CD14⁺ monocytes were exposed to medium alone (top row), 100 nM Trx80 (middle row), or GM-CSF + IL-4 (bottom row) and cultured for 3 and 5 days in ultralow attachment plates. For triple marker staining, cells were incubated with a combination of FITC-conjugated CD83, PE-conjugated CD1a, and APC-conjugated CD14 and analyzed by flow cytometry. The proportion of positive cells is indicated. One representative experiment of 5 is shown.