Trx80 induces phosphorylation of p38, ERK, and JNK in TAMs. TAMs were produced by exposure of CD14⁺ monocytes to 100 nM Trx80 for the time periods indicated. The levels of phosphorylation of p38 (A, top), ERK (B, top), and JNK kinase (C, top) were determined by Western blotting of whole-cell lysates using antibodies specific for the phosphorylated, activated forms. The corresponding Western blots for the total levels of these kinases are depicted in the bottom panels. One representative experiment is shown.