Ex vivo generation of HA-1-specific cytotoxic T cells from HLA-A2^pos/HA-1^neg cord blood. Purified CB CD8^pos T cells were cultured in the presence of autologous HA-1^H-expressing dendritic cells and T helper cells. Results of 2 different CB cultures are shown (I and II). Samples were stained with HA-1^A2 tetramers (x-axis) and CD8 antibodies (y-axis). The percentages of HA-1^A2 tetramer^pos CD8^pos T cells are shown after 14 (A, B), 28 (C), or 21 days of culture (D). HA-1^A2 CD8^pos T cells were FACS sorted (indicated by arrow), expanded, and tested for cytoxic activity (E, F). Target cells: HLA-A2^pos/HA-1^neg EBV-LCL (•), HLA-A2^pos/HA-1^neg EBV-LCL pulsed with HA-1^H peptide (▪), and HLA-A2^pos/HA-1^pos EBV-LCL (▴). Data are presented as mean percentage of lysis ± SD.