Influence of RGS2 on Flt3-ITD–induced signaling pathways. The 32D cells stably transfected with either Flt3-WT or Flt3-ITD together with a control vector or with Flt3-ITD together with RGS2 were starved of growth factors for 12 hours and were subsequently exposed to the indicated cytokines for 10 minutes. Western blot analyses were performed with lysates from these cells using phospho-specific antibodies for Akt (A), Gsk3-β (B), or STAT5 (C). The membranes were reprobed with antibodies detecting total proteins.