Figure 2.
“Cross-phenotyping” of IL-3Rα+ and IL-3Rα– BM progenitors for CD34 and FcγR expression. Lin–c-Kit+Sca-1–IL-7Rα– cells (black gate in panel B) were stained for CD34 versus FcγR expression and resolved (E) into CD34+FcγRlo (CMP), CD34+FcγRhi (GMP), and CD34–FcγRlo (MEP).6 For comparison, c-Kit+lin–Sca-1–IL-7Rα– cells were sorted into IL-3Rα– (A) and IL-3Rα+ (C) subsets, and then restained for CD34 and FcγR expression. c-Kit+IL-3Rα– cells include MEP and CMP phenotypes (D), and c-Kit+IL-3Rα+ cells include GMP and CMP phenotypes (F).