Inhibition of wild-type and mutant Bcr-Abl by imatinib and PD166326.Point mutations of Bcr-Abl that were identified in resistant colonies were cloned. Parental Ba/F3 cells were transfected with mutant constructs. Stable cell lines were incubated without (w.o.) and in the presence of imatinib (left column) and PD166326 (right column) at the indicated concentrations. Whole cell lysates were subjected to SDS-PAGE. Blots were probed for phosphotyrosine and Abl. Y253H, E255K, E255V, and T315I were previously reported.¹⁸