Increase in ERP5 cell-surface expression in a concentration- and time-dependent manner in response to platelet stimulation by convulxin, collagen, or thrombin. (A) Concentration dependence with 90-second stimulation. (i) Stimulation by convulxin (Cvx). Basal level is indicated by filled histogram; stimulation with 10 ng/mL Cvx, by an empty histogram; and stimulation with 40 ng/mL Cvx, by an empty dotted-line histogram. (ii) Stimulation with collagen (Coll). Basal level is indicated by a filled histogram; stimulation with 25 μg/mL Coll, by an empty histogram; and stimulation with 100 μg/mL Coll, by an empty dotted-line histogram. (iii) Stimulation with thrombin (Thr). Basal level is indicated by filled histogram; stimulation with 0.2 U/mL Thr, by an empty histogram; and stimulation with 1.0 U/mL Thr, by an empty dotted-line histogram. (B) Time dependence. (i) Basal level is indicated by filled histogram; 45-second stimulation with 40 ng/mL Cvx by an empty histogram; and 300-second stimulation with 40 ng/mL Cvx, by an empty dotted-line histogram. (ii) Stimulation by Coll. Basal level is indicated by a filled histogram; 45-second stimulation with 25 μg/mL Coll by an empty histogram; and 300-second stimulation with 25 μg/mL Coll, by an empty dotted-line histogram. (iii) Stimulation by Thr. Basal level is indicated by a filled histogram; 45-second stimulation with 1.0 U/mL Thr, by an empty histogram; and 300-second stimulation with 1.0 U/mL Thr, by an empty dotted-line histogram. (C) Normalized plots for the increase in cell-surface exposure observed for ERP5 over time are given for convulxin (i), collagen (ii), and thrombin (iii). Data are presented as mean ± standard error (SE) (n = 3), where 100% represents the maximal response detected.