Figure 1.
SAP expression reflects the presence of activated T cells. Immunoblot of lysates corresponding to 1 × 105 cells. In all experiments, Jurkat and LCLs were used as positive and negative controls, respectively. (A) T-enriched cells from cord blood were exposed to PHA for 3 days. Dose response. (B) EBV-infected CBMCs cultured with 10 μg/mL, 25 μg/mL, and 50 μg/mL PSK. (C) Cultures of CBMCs were initiated with or without EBV infection. Trx80 (100 nM), PSK (25 μg/mL), or Trx80 + PSK were added as indicated. The results show 1 of 4 experiments. (D) To the EBV-infected CBMC cells Trx80, IL-2 (20 U/mL), or both were added as indicated. Panels B-D represent results of 6-day-old cultures.
