Cytokine production in the PSK- or Trx80-containing CBMC cultures. (A-B) IL-15 and IL-12 content in the supernatant of 3-day-old cultures tested by ELISA. Cultures with Trx80, PSK, or Trx80 + PSK are marked as T, P, and T + P, respectively. (A) EBV-infected CBMCs. The results represent mean ± SD of 3 independent experiments. (B) The monocyte-depleted population was infected with EBV. (i,iii) Cultured without or with Trx80, PSK, or Trx80 + PSK. (ii,iv) The isolated monocytes were exposed for 20 hours without or with Trx80, PSK, or Trx80 + PSK and reintroduced to the cultures. The results represent mean ± SD of 4 independent experiments. (C) Intracellular expression of IL-15 and IL-12 detected by flow cytometry. EBV-infected CBMCs were cultured alone or with Trx80 or PSK for 48 hours. During the final 10 hours, monensin was added. Panels Ci-iii and Cv-vii show IL-15 or IL-12 expression of the CD14⁺ monocytes in the gated large cell population. Panels Civ and Cviii show IL-15 or IL-12 expression of the CD3⁺ T cells in the gated small populations. The results show 1 of 3 independent experiments with similar results.