3H3 treatment inhibits the production of IgG₁ anti-DNA autoantibody and total IgE in cGVHD. cGVHD was induced by transferring 8 × 10⁷ DBA/2 spleen/lymph node cells into BDF₁ mice. Immediately thereafter, 200 μg of 3H3 (▪) or control Ig () was injected. Serum samples were collected every 2 weeks and assayed in duplicate by ELISA for IgG₁ anti-DNA autoantibody (B) and total IgE (D). The optical density (OD) of duplicate samples for each mouse was measured at 450 nm, using serially diluted serum samples. Titration curves for anti-DNA IgG₁ are shown in panel A and those for total IgE at week 2 in panel C. OD values are means ± SD (n = 10 per group) of 10-fold dilutions of samples and are representative of more than 3 independent experiments. ^*P < .05, between the 2 groups at the indicated time points.