CD4⁺CD25⁺CD62L⁺ and CD4⁺CD25⁺CD62L^- T cells express comparable levels of GITR on their surface but differ in the expression of other markers. C57BL/6 splenocytes were stained with anti-CD4 Cy7APC, anti-CD25 APC, anti-CD62L FITC, and PE-labeled antibody against the marker of interest and analyzed by flow cytometry. Two-dimensional dot plots of CD62L versus GITR, CD44, CD45RB, or CD69 are shown for CD4⁺CD25^- T cells in the left and CD4⁺CD25⁺ T cells in the middle column. The histograms in the right column are overlays of the respective markers for CD4⁺CD25^- T cells (shaded), CD4⁺CD25⁺CD62L^- (bold), and CD4⁺CD25⁺CD62L⁺ Treg cells (fine). The gates for CD4⁺CD25^- and CD4⁺CD25⁺ cells as well as the CD62L+ and CD62L- subsets are shown in the two top FACS plots.