CD4⁺CD25⁺CD62L⁺ T cells home significantly better to secondary lymphoid tissues than CD4⁺CD25⁺CD62L^- T cells. Sorted CD4⁺CD25⁺CD62L⁺ (▨) and CD4⁺CD25⁺CD62L^- T cells (▪) were labeled with ¹¹¹In before cotransfer with unlabeled CD4⁺CD25^- T cells and TCD BM into irradiated hosts. Mice were killed 48 hours later and radioactivity in the various organs was measured. (A) Global comparison of aGVHD target organs (liver, small bowel, large bowel) and primary lymphoid organs (spleen, peripheral LN [PLN], mesenteric LN [MLN]). Results are expressed as fraction of injected radioactive dose divided by organ weight in grams (% ID/g) representing organ-specific enrichment. Mean + SD is given. (B) Results from individual mice are shown for mesenteric LN, spleen, and liver. The horizontal line represents the group median. P values are provided above the figure. Data were pooled from 3 experiments (CD4⁺CD25⁺CD62L⁺ mice, n = 7; CD4⁺CD25⁺CD62L^- mice, n = 8).