American Society of Hematology

Figure 1.

$Figure 1. Lack of significant AF-specific T-cell responses by CFC in healthy donors. PBMCs from healthy CMV-seropositive donors were stimulated with SEB, CMV lysate, and AF lysate or left unstimulated (US) and analyzed by CFC by sequential gating of lymphocytes, CD4+ T cells, and TNFα CD69+ cells. Stimulation with SEB and CMV lysate resulted in the expected fraction of functional cells with an average of 12.7% and 1.82%, respectively (US vs SEB, P = .0002; US vs CMV, P = .0002). Incubation of cells with Aspergillus fumigatus lysates resulted in frequencies of activated cells comparable to that seen in the unstimulated control sample (mean of 0.19% vs 0.16% in the US; US vs AF, P = .64). Similarly, we failed to detect cytokine production in AF-stimulated CD8+ T cells (not shown). Horizontal bars indicate means.$

Lack of significant AF-specific T-cell responses by CFC in healthy donors. PBMCs from healthy CMV-seropositive donors were stimulated with SEB, CMV lysate, and AF lysate or left unstimulated (US) and analyzed by CFC by sequential gating of lymphocytes, CD4⁺ T cells, and TNFα CD69⁺ cells. Stimulation with SEB and CMV lysate resulted in the expected fraction of functional cells with an average of 12.7% and 1.82%, respectively (US vs SEB, P = .0002; US vs CMV, P = .0002). Incubation of cells with Aspergillus fumigatus lysates resulted in frequencies of activated cells comparable to that seen in the unstimulated control sample (mean of 0.19% vs 0.16% in the US; US vs AF, P = .64). Similarly, we failed to detect cytokine production in AF-stimulated CD8⁺ T cells (not shown). Horizontal bars indicate means.

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