Confirmation of GT-induced apoptosis by annexin V staining. Healthy donor PBMCs were separated into CD14⁺ (monocyte, ▪) and CD14- (lymphocyte, □) fractions using a magnetic bead cell selection system. Separated cells were incubated with 35 ng/mL GT for 1, 2, 4, and 6 hours. The percentage of apoptotic cells within the monocytes increased from 6.4% after 1 hour to 50.4% by the end of a 6-hour incubation. Apoptosis was significantly lower in lymphocytes than in monocytes, consistent with results obtained using caspase-3 induction as a marker of apoptosis. Error bars indicate standard deviation.