GT induces apoptosis in CD83⁺ dendritic cells. mDCs were incubated for 3 hours with or without 35 ng/mL GT and analyzed by flow cytometry by sequential gating on DC, CD83-positive cells, and active caspase-3–positive cells. Panel A depicts a control sample with no GT added to the culture. Panel B depicts that same sample with GT added. Panels C and D display the percentage of caspase-3–positive cells within the same cell populations as in panels A and B, respectively. Apoptosis, as evidenced by caspase-3 positivity, in DCs incubated with GT was increased more than 7-fold over the control (20.5% vs 2.8%, respectively).