H-2g induces HMVEC VEGF, and bFGF through JAK2, PI3K, and NFκB. HMVECs were pretreated with chemical inhibitors: AG490, LY294002, and PD98059. HMVECs were then exposed to H-2g (100 nM) for 12 hours. ELISAs of the supernatants showed that H-2g induced both VEGF (A) and bFGF (B) expression and that the JAK2 inhibitor, AG490, or the PI3K inhibitor, LY294002, inhibited H-2g–induced EC VEGF and bFGF. Next, HMVECs were transiently transfected with JAK2, PI3K, Erk1/2 antisense ODN, or control sense ODN, or with decoy NFκB or scrambled NFκB. At 16 hours after transfection, HMVEC VEGF and bFGF ELISAs were performed. Decoy NFκB and antisense JAK2 and PI3K ODNs (□) inhibited HMVEC VEGF and bFGF expression compared with their control ODNs (▪) (C-D). Results are expressed as the mean number of cells migrating through the membrane per well plus or minus SEM from 4 independent experiments. ^*Represents a significant difference (P < .05) between the respective groups.