Figure 5.
VE-cadherin antibody-mediated blood vessel disassembly does not involve alteration of mesodermal or endothelial subpopulations. (A-B) Representative FACS profiles of cells isolated from control and BV13-treated (24 μg IgG/mL) 8.5-dpc cultured allantoides, respectively. (C) A graphic compilation of the FACS data from 4 experiments comparing control versus BV13-treated cultures. Together these analyses show no significant changes in the Flk1+ (angioblasts; filled bars), Flk1+/PECAM+ (endothelial cells; dark gray bars), or propidium iodide+ (dead cells; light gray bars) cell populations in BV13-treated allantois cultures as compared to control cultures. Error bars indicate the standard deviation of the mean.