NY-ESO-1 formulations induce distinct antigen-presentation kinetics. MoDCs were pulsed with NY-ESO-1 protein (), NY-ESO-1/IC (□), or NY-ESO-1/IMX (•; 10 μg/mL for MHC I and 1 μg/mL for MHC II assays) or peptide (○) for 1 hour, washed, and activated with CD40L. After various time points, DCs were cocultured with NY-ESO-1–specific CD8⁺ T cells (A) or CD4⁺ T cells (B). T-cell IFN-γ production was quantified by ICS. Data are representative of 4 experiments.