Lack of cross-presenting function of plasmacytoid DCs persists after activation with maturation-inducing stimuli. (A) Surface expression of CD86 and MHC I by plasmacytoid DCs following culture with IL-3 in the absence or presence of CpG ODN2006, ODN2216, or R-848. (B) Freshly isolated plasmacytoid DCs were pulsed with NY-ESO-1/IC (10 μg/mL), matured with the indicated stimuli, and cocultured with NY-ESO-1–specific CD8⁺ T cells (left graph). NY-ESO-1/IC–pulsed MoDCs served as a positive control (right graph). IFN-γ production by CD8⁺ T cells was quantified by ICS. □ indicates DCs only; ▪, + peptide; ▦, + NY-ESO-1/IC. Data are representative of 5 experiments.