Knockdown of vcyclin and vFLIP in PEL cells. (A) Graph shows the percent of PEL cells remaining viable as determined by FACS analysis for annexin V-FITC 10 days after infection with sh-vcyclin or sh-vFLIP. □ indicates JSC-1 cells; ▦, BC-3; ▩ , BCP-1; and ▨, HBL-6. (B) Light microscopy (magnification 100 ×) of BC-3 cells 10 days after infection with sh-LANA, sh-vcyclin, or sh-vFLIP shows extensive cell loss with vcyclin or vFLIP knockdown. (C) Graph shows percent of BC-3 cells remaining viable as determined by FACS analysis for annexin V-FITC at timepoints after infection with sh-LANA (▦), sh-vcyclin ( ▩ ), or sh-vFLIP (▨)atanMOI of 10 293t IU/cell. Error bars indicate the standard error of the mean.