In vivo model for PEL. (A) Graph of weight gain of a group of 10 mice over the course of 21 days after intraperitoneal inoculation with 1 × 107 JSC-1 cells. (B) LANA IFA of ascitic fluid drained from mice 14 days after inoculation with JSC-1 cells. LANA-positive cells (blue arrow) show typical nuclear stippling and indicate reliable development of appropriate ascites, while the presence of small cells (red arrows), which stain negative for LANA, are murine cells in the ascitic tap. (C) Graph shows the weight gain of 3 groups of 6 mice each: weight-only control group ( ▩ ), IP co-injection with 1 × 107 JSC-1 cells, and 1 × 108 293t IU of empty vector (▦) or sh-vcyclin (□). Outliers marked on the sh-vcyclin group represent 2 treated mice that developed ascites despite administration of sh-vcyclin. Outliers were included in all statistical analyses. Error bars indicate the standard error of the mean.
