American Society of Hematology

Figure 4.

$Figure 4. Loss of monolayer integrity and cell junction proteins after MRP8/MRP14 treatment. HMEC monolayers were cultured on coverslips and were treated for 16 hours with MRP8/MRP14 or control medium. Immunofluorescence staining was performed for membranous structures with filipin (A) and for VE-cadherin (B), β-catenin (C), and ZO-1 (D). Loss of cell junction proteins and substantial holes are indicated by arrows (A-D). Scale bar, 10 μm. Occludin (E), ZO-2 (F), β-catenin (G), and ZO-1 (H) were analyzed in postnuclear supernatants (P), cytosol (C), and membrane (M) fractions of whole cell lysates of MRP8/MRP14-treated and control HMECs by immunoblotting of equal amounts of protein.$

Loss of monolayer integrity and cell junction proteins after MRP8/MRP14 treatment. HMEC monolayers were cultured on coverslips and were treated for 16 hours with MRP8/MRP14 or control medium. Immunofluorescence staining was performed for membranous structures with filipin (A) and for VE-cadherin (B), β-catenin (C), and ZO-1 (D). Loss of cell junction proteins and substantial holes are indicated by arrows (A-D). Scale bar, 10 μm. Occludin (E), ZO-2 (F), β-catenin (G), and ZO-1 (H) were analyzed in postnuclear supernatants (P), cytosol (C), and membrane (M) fractions of whole cell lysates of MRP8/MRP14-treated and control HMECs by immunoblotting of equal amounts of protein.

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