Figure 4.
Figure 4. MSC-mediated inhibition of T-cell proliferation is irreversible. C6 splenocytes (5 × 106) were stimulated with syngeneic KkSmcy HY peptide (TENSGKDI)–pulsed APCs (Ag; 5 × 106) in the presence or absence of MSCs (1 × 105). After 24 or 48 hours, MSCs were removed from the culture by immunodepletion using anti-CD106 mAb, and the T cells (1 × 105) were restimulated with 10 IU/mL IL-2 (▨), or with IL-2 and 5 × 105 HY peptide–pulsed APCs (□), or HY peptide–pulsed APCs only (▪). T-cell proliferation was measured by 3H-Tdr incorporation on day 3. Results are the average of 3 experiments of identical design; bars show the SD. *Statistically significant (P < .01).

MSC-mediated inhibition of T-cell proliferation is irreversible. C6 splenocytes (5 × 106) were stimulated with syngeneic KkSmcy HY peptide (TENSGKDI)–pulsed APCs (Ag; 5 × 106) in the presence or absence of MSCs (1 × 105). After 24 or 48 hours, MSCs were removed from the culture by immunodepletion using anti-CD106 mAb, and the T cells (1 × 105) were restimulated with 10 IU/mL IL-2 (▨), or with IL-2 and 5 × 105 HY peptide–pulsed APCs (□), or HY peptide–pulsed APCs only (▪). T-cell proliferation was measured by 3H-Tdr incorporation on day 3. Results are the average of 3 experiments of identical design; bars show the SD. *Statistically significant (P < .01).

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