Cell growth pattern and FACS analysis. (A) Immunophenotype of the expanded clonal cells. The solid line outlines the region of fluorescent intensity for cells labeled with control antibodies. The dotted lines indicate the region of fluorescent intensity for cells labeled with specific antibodies. (B) The expanded clonal cells proliferated with a population doubling time of about 32 hours during the log phase of growth. (C) These expanded clonal cells did not express GATA-1 or GATA-2 genes but expressed SCL gene and BCR/ABL fusion gene. Lanes 1, 2, and 3 represent positive control, negative control, and experimental samples, respectively.