Immunoneutralization of RANTES reduces T-cell migration in vitro and the severity of IPS after allogeneic SCT. (A) Freshly isolated splenic T cells were allowed to migrate to RANTES through transwell inserts (5-μm pores) for 4 hours in the presence (▦) or absence (▪) of polyclonal antibodies against RANTES and compared to basal migration without a chemokine stimulus (□). Data presented are from 1 of 3 comparable experiments; ^*P < .05. Lethally irradiated B6D2F1 mice received allogeneic wild-type SCT and either control serum (▪) or anti-RANTES polyclonal antibodies (▦). Syngeneic animals received control serum only (□). Lung injury was assessed by pathology score (B) and BALF cellularity (C) 6 weeks following allo-SCT. Data are presented as mean ± SEM and are pooled from 2 similar experiments; n = 6 to 8 per group; ^*P < .05.