Effects of signaling inhibitors on expression of MIG induced by RANKL. Osteoclast precursors were pretreated with 30 μg/mL SN50, 20 μM SB203580, 1 μM PD98059, or 20 μM SP600125 for 30 minutes and were stimulated in the presence of 50 ng/mL M-CSF plus 100 ng/mL RANKL for 12 hours. Expression levels of MIG were analyzed by RT-PCR (A) or Western blot analysis (B).