Lack of MIG induction by RANKL in STAT1-deficient osteoclast precursors. Osteoclast precursors from wild-type and STAT1-deficient mice were stimulated with 50 ng/mL M-CSF plus 100 ng/mL RANKL for the indicated time. RT-PCR was performed with isolated RNA using primers specific for each of MIG, MMP-9, Fra II, and GAPDH. RT-PCR products were separated on a 1.2% agarose gel and were stained with ethidium bromide.