Premature death and pleural effusion in kCYC^+/- transgenic mice. (A) Kaplan-Meier survival curves for transgenic (TG) mice (solid line, n = 77) and WT littermates (dotted line, n = 94). (B) A 3-dimensional reconstituted CT image of a TG mouse and a WT littermate with numbers showing air space content. (C) Chylothorax detected by water-enhanced MR images. (D) Chylous fluid (arrow) in pleural cavity following removal of the lungs. (E) H and E staining of lung tissue showing lymphocytes contained within vascular structures near blood vessels (arrows) (× 100). (F) Anti-VEGFR-3 for lymphatics (red) and DAPI (4,6 diamidino-2-phenylindole) staining for nuclei (blue) in lung tissue showing lymphatics filled with cells (× 100).