Immunophenotypic analysis of cord blood EPC-derived endothelial cells, HUVECs, and HAECs. Immunophenotyping of cell monolayers derived from cord blood EPCs (A), umbilical veins (B), or human aortas (C) by fluorescence cytometry. Cord blood EPC-derived ECs, HUVECs, and HAECs express CD31, CD141, CD105, CD146, CD144, VWF, and Flk-1 but do not express CD45 and CD14. Shown are representative data from 5 independent experiments using 5 different cord blood EC monolayers, 5 different HUVEC samples, and 5 different HAEC samples with similar results. Isotype controls are overlaid in white on each histogram for each surface antigen tested.