RAPA blocks cell cycle in G1 phase and induces p27kip1 in KG1a cells. (A) KG1a cells were incubated with or without RAPA (10 nM) for 24 hours and then processed for cell cycle analysis. (B) KG1a cells (106 cells) were incubated with or without RAPA (10 nM) for 4 and 24 hours, lysed, and analyzed by Western blotting with the indicated antibodies. Results shown are representative of 3 independent experiments.